The service provides predictions of genetic structures based on neural network algorithms and Markov statistical models. It analyzes data about the frequency of amino acid sequences for various organisms and generates likely existence and locations of signal peptide cleavage sites to be expected in their genetic structures. Organism groups covered include gram-positive and gram-negative prokaryotes as well as eukaryotes. The same provider maintains an [[http://www.cbs.dtu.dk/ws/ws.php?entry=SignalP4 alternative service]], as well.
API methods support submission of the amino acid sequence to be analyzed along with an organism type, preferred prediction method, and desired statistical prediction certainty threshold. Methods also support monitoring the service processing queue and retrieving the completed analytical result.